Table 1.

Strains and plasmids used in this work

Strain or plasmid	Relevant genotype/phenotypea	Source or reference
E. coli
DH5α	F− ϕ80lacZ ΔM15 Δ(lacZYA-argF)U169 deoP recA1 endA1 hsdR17 (rk− mk−)	Invitrogen
S17-1λpir	recA thi pro hsdR hsdM+ RP4::2-Tc::Mu::Kan Tn7 λ pir lysogen, Tpr Strr	61
Y. enterocolitica
JB580v	8081v (r− m+ Nalr) serotype O:8, biovar 1B	62
YVM1334	JB580v with deletion of yaxAB	This work
MC5	Clinical isolate, biovar 1A	Gift from M. Cafferkey
MC7	Clinical isolate, biovar 1A	Gift from M. Cafferkey
MC8	Clinical isolate, biovar 1A	Gift from M. Cafferkey
WC-A	Biovar 1B	63
Y295	Biovar 1B	64
657-83	Reference strain, biovar 1B	65
9291-78	Clinical isolate, biovar 1B	65, 66
2400-87	Biovar 1B	64
MC22	Clinical isolate, biovar 3	67
MC32	Clinical isolate, biovar 3	64, 67
MC33	Clinical isolate, biovar 3	67
MC34	Clinical isolate, biovar 3	67
MC6	Clinical isolate, biovar 4	67
MC28	Clinical isolate, biovar 4	67
MC51	Clinical isolate, biovar 4	64, 67
Plasmids
pSR47S	MobRP4 oriR6K cloning vector, Kanr	68
pGK501	yaxA/B deletion in pSR47S, Kanr	This work
pMWO-005	Low-copy cloning vector, Kanr	39
pMWO-034	Low-copy cloning vector, Kanr	39
pNW306	yaxA and yaxB coding sequence in pMWO-034, Kanr	This work
pNW307	yaxA-myc and yaxB-HA coding sequence in pMWO-034, Kanr	This work
pNW311	yaxA-myc coding sequence in pMWO-034, Kanr	This work
pNW312	yaxB-HA coding sequence in pMWO-005, Kanr	This work

^aTp^r, trimethoprim resistant; Str^r, streptomycin resistant; Kan^r, kanamycin resistant; Nal^r, nalidixic acid resistant.