Table 2.
Primers used in this work
| Primer name | Sequencea (5′-3′) |
|---|---|
| YE1984/5del1 | GCGTCGACGTTTTATGGCTGATTTAAATTTC |
| YE1984/5del2 | CGGGATCCTTGTGTCATTGTATTATCCTCATC |
| YE1984/5del3 | CGGGATCCCAAACAGCTGATTTGACTTGTG |
| YE1984/5del4 | ATAAGAATGCGGCCGCCTTCACTTGTGACTCCATCTC |
| Yax001 | GTGCGTCGACGTCTTCTTACCTTTACGGGTCTGC |
| Yax004 | CGGAATTCCGCCTACCTTTACGGGTCTGC |
| Yax005 | CGGGATCCCGAAATCAGCTGTTTGATTAATGA |
| Yax006 | CGGGATCCCGCGAGCGATGTCTGGGACGTCGTATGG GTAAATCAGCTGTTTGATTAATGAC |
| Yax009 | TTACAGATCTTCTTCAGAAATAAGTTTTTGTTCGCCATACACTTTTTTGTATTC |
| Yax010 | GAACAAAAACTTATTTCTGAAGAAGATCTGTAATCAATATTACCGTATTTAATTATTC |
| Yax011 | GGAATTCCTCATAATGAATAATTAAATACG |
| Yax013 | GGGTACCCCGGAAATAAGCACATTTCCA |
| Yax014 | GTGCGTCGCACACAAACACAATTGGCTATTG |
| Yax015 | TGAAGCGGCCGCTCAAATCAGCTGTTTGATTAATGAC |
| MWO-05rev | CAGCCTGAAACAGGCGATGCTGC |
a
Restriction sites are underlined, and sequences for epitope tags are in boldface.