Contribution of MyD88, TRIFF, and IRAK4 in TLR4 signaling. (A) MyD88, TRIFF, and IRAK4 knockdown was assessed by QPCR (n = 2). (B) Secretion of IL-6, IL-8, CXCL10, and CCL5 by CT or MyD88, TRIFF, and IRAK4 knockdown meningothelial cells after contact with meningococci (strain MC58) or LPS stimulation (n = 4). ANOVA was followed by Dunnett's multiple-comparison test.