Fig 8.

Role of NF-κB p65, p38 MAPK, JNK1, IRF3, and IRF7 transcription factors in meningothelial cell stimulation. (A) Transcription factor phosphorylation. Meningothelial cells were stimulated with MC58. At the indicated times, proteins were collected and analyzed by Western blotting for NF-κB p65, p38 MAPK, JNK1, IRF3, and IRF7 phosphorylation. The results of 1 experiment representative of 3 are shown. (B) Role of NF-κB p65, p38 MAPK, and JNK1 transcription factors in the meningothelial cell response to meningococci. Meningothelial cells were treated with inhibitors of NF-κB p65 (Bay 11-7082; BAY), p38 MAPK (SB203580; SB), or JNK1 (SP600125; SP) for 1 h before stimulation with meningococci (strain MC58). IL-6, IL-8, CXCL10, and CCL5 secretion was then measured (n = 2).