Table 2.
Effect of TLR4, MyD88, TRIF, and IRAK4 knockdown on cytokine productiona
| Condition | % cytokine production |
|||||
|---|---|---|---|---|---|---|
| MC58 |
LPS |
|||||
| IL-6 | CXCL10 | CCL5 | IL-6 | CXCL10 | CCL5 | |
| CT | 100 | 100 | 100 | 100 | 100 | 100 |
| TLR4− | 14 ± 4 | 11 ± 3 | 3 ± 3 | 9 ± 3 | 4 ± 2 | 3 ± 3 |
| Myd88− | 24 ± 1 | 49 ± 6 | 37 ± 14 | 19 ± 5 | 47 ± 13 | 38 ± 12 |
| Trif− | 51 ± 11 | 31 ± 15 | 34 ± 15 | 43 ± 10 | 28 ± 14 | 47 ± 9 |
| IRAK4− | 75 ± 6 | 75 ± 2 | 83 ± 10 | 75 ± 11 | 84 ± 10 | 98 ± 4 |
a
The percentage of cytokine production after stimulation with MC58 or LPS, compared with that for CT cells, was calculated for each condition.