Fig 2.

Salmonella requires ferric iron (fepB) for growth in macrophages. (A) Cell culture macrophages (Nramp1⁺) were infected with the wild-type, ΔfepB, or fepB-restored (fepB⁺) strain at a multiplicity of infection of 10. At 2, 18, and 24 h postinfection, macrophages were lysed, serially diluted, and plated to determine numbers of CFU/ml. Error bars indicate standard errors of the means (n ≥ 3 experiments). ∗, P < 0.05 versus the wild type. (B) Cell culture macrophages (Nramp1⁺) were infected with the wild type in the absence or presence of 50 μM deferasirox at a multiplicity of infection of 10. At 2, 18, and 24 h postinfection, macrophages were lysed, serially diluted, and plated to determine numbers of CFU/ml. Error bars indicate standard errors of the means (n ≥ 3 experiments). ∗, P < 0.05 versus the wild type.