Fig 3.

Salmonella can grow with ferric iron as the sole iron source. (A) The indicated strains were grown in LB medium supplemented with 200 μM iron chelator 2,2′-dipyridyl (dipi). The optical density at 600 nm was monitored for 16 h. Error bars indicate standard deviations (n ≥ 3 experiments). ∗, P < 0.05 versus the wild type; ∗∗, P < 0.05 versus the ΔfepB mutant. (B) The indicated strains were grown in M9 minimal medium supplemented with ferric chloride (Fe³⁺) and/or 200 μM dipyridyl. The optical density at 600 nm was monitored for 16 h. Error bars indicate standard deviations (n ≥ 3 experiments). ∗, P < 0.05 versus the wild type; ∗∗, P < 0.05 versus the ΔfepB mutant.