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Thermal shift assay showing that SE-1 binds to VirF protein. The thermal denaturation of MBP-VirF (A) and MBP-NS1-NTD (B) was measured using the dye Sypro Orange. Assays were carried out in the absence (DMSO only) or presence of 80 mM SE-1. Readings were taken from 25 to 99°C. Only the relevant portions of the curves are shown. Results of a single experiment (representative of three independent assays with two replicates each) are shown.
