Table 1.
PCR primers used for quantification of gene expression
| Protein | Gene | Orientationa | Oligonucleotide sequence (5′–3′) | Amplicon size (bp) |
|---|---|---|---|---|
| Hemolysin-adenylate cyclase | cyaA | F | CGAGGCGGTCAAGGTGAT | 93 |
| R | GCGGAAGTTGGACAGATGC | |||
| Virulence factor transcription regulator | bvgA | F | AGGTCATCAATGCCGCCA | 140 |
| R | GCAGGACGGTCAGTTCGC | |||
| Filamentous hemagglutinin | fhaB | F | CAAGGGCGGCAAGGTGA | 120 |
| R | ACAGGATGGCGAACAGGCT | |||
| RNA polymerase subunit beta | rpoB | F | GCTGGGACCCGAGGAAAT | 95 |
| R | CGCCAATGTAGACGATGCC | |||
| Pertussis toxin subunit 1 | ptxA | F | CCAGAACGGATTCACGGC | 112 |
| R | CTGCTGCTGGTGGAGACGA | |||
| Pertussis toxin transport protein A | ptlA | F | CCCTTGAAAGACTTGAGAGCATC | 154 |
| R | GCCGCGCAGTACGACCA | |||
| Pertussis toxin transport protein F | ptlF | F | TGTGCTGTATATCAAGGCCAAAT | 96 |
| R | GTTTTGACGAGCAGATTGGTGT | |||
| Pertussis toxin transport protein G | ptlG | F | CACGTTTATCGATTGCATCCTG | 140 |
| R | GTATTCGCCCACCACTGTCG | |||
| Pertussis toxin transport protein H | ptlH | F | ATATAGCGGAAATCTGCGTGAA | 150 |
| R | GGCAAATGTCGCGGTCG |
a
F, forward primer; R, reverse primer.