Table 1.
Strain or plasmid | Descriptiona | Source or reference |
---|---|---|
Strains | ||
KIM6+ | Tc genes present, pCD1 negative, pgm positive, pPCP1 positive | 51 |
KIM6 | Tc genes present, pCD1 negative, pgm negative, pPCP1 positive | 51 |
KIM10 | Tc genes present, pCD1 negative, pgm negative, pPCP1 negative | 9 |
KIM5 | Tc genes present, pCD1 positive, pgm negative, pPCP1 positive | 52 |
KIM6+ ΔyitA-yipB | Deletion of the Tc locus from yitA to yipB, yitR still present | 8 |
KIM6+ ΔyitR | Deletion of the regulator YitR | 7 |
KIM6+ YitA–β-lactamase | YitA::β-lactamase translational fusion | 8 |
KIM6+ YipA–β-lactamase | YipA::β-lactamase translational fusion | 8 |
KIM5 YitA–β-lactamase | YitA::β-lactamase translational fusion | 8 |
KIM5 YipA–β-lactamase | YipA::β-lactamase translational fusion | 8 |
KIM6 YitA–β-lactamase | YitA::β-lactamase translational fusion | 8 |
KIM6 YipA–β-lactamase | YipA::β-lactamase translational fusion | 8 |
Plasmids | ||
pCR-XL-TOPO::yitR | yitR expressed under native promoter control from a high-copy-number plasmid (Kan, Zeo) | 8 |
pUC19::yitR | yitR expressed under native promoter control from a high-copy-number plasmid (Amp) | This study |
pWKS130::yitR | yitR expressed under native promoter control from a low-copy-number plasmid (Kan) | 7 |
pCD1Δ1234 | Plasmid encoding the genes required for the T3SS, with no effector Yops (Cam) | 18 |
pCD1::kan | pCD1ΔyadA::kan (Kan) | 53 |
pMM83 | Plasmid pHSG576 encoding YopM–β-lactamase under control of its native promoter (Cam) | 32 |
pMM85 | Plasmid pHSG576 encoding YopE–β-lactamase under control of its native promoter (Cam) | 32 |
pAcGFP1 | Constitutively produces GFP (Amp) | Clontech |
pmCherry | Constitutively expresses mCherry (Amp) | Clontech |
Antibiotic resistances, where present, are noted in parentheses. Kan, kanamycin; Amp, ampicillin; Cam, chloramphenicol; Zeo, zeocin.