Table 2.
Transmission of KIM6+(pCD1Δ1234) and KIM6+ ΔyitA-yipB(pCD1Δ1234) from coinfected fleas in two independent experiments
| Combined no. of CFU/ml in blood meala | Initial ratiob | Replicate | Strainc | No. of CFU transmittedd on day: |
Total no. of CFU transmitted | Transmission ratioe | ||
|---|---|---|---|---|---|---|---|---|
| 14 | 21 | 28 | ||||||
| 3.00e9 | 1.39 | 1 | Tc+ | 0 | 1,218 | 102 | 1,320 | 1.43 |
| 1 | Tc− | 2 | 598 | 73 | 673 | |||
| 2 | Tc+ | 5 | 1,344 | 335 | 1,684 | |||
| 2 | Tc− | 3 | 1,085 | 334 | 1,422 | |||
| 5.75e7 | 1.65 | 1 | Tc+ | 129 | 113 | 14,040 | 14,282 | 1.56 |
| 1 | Tc− | 75 | 52 | 8,940 | 9,067 | |||
| 2 | Tc+ | 15 | 0 | 262 | 277 | |||
| 2 | Tc− | 9 | 0 | 232 | 241 | |||
a
Total number of CFU/ml of KIM6+(pCD1Δ1234) and KIM6+ ΔyitA-yipB(pCD1Δ1234) in the infectious blood meal.
b
Ratio of KIM6+(pCD1Δ1234) CFU to KIM6+ ΔyitA-yipB(pCD1Δ1234) CFU in the infectious blood meal.
c
Tc+, KIM6+(pCD1Δ1234); Tc−, KIM6+ ΔyitA-yipB(pCD1Δ1234).
d
Number of CFU of each strain transmitted in two independent flea feedings of two different groups of fleas for each experiment.
e
Ratio of cumulative number of Tc+ CFU to cumulative number of Tc− CFU transmitted.