Fig 7.

c-Jun and Ets1 directly bind to the ras-responsive element on the p38δ promoter. Chromatin was immunoprecipitated from BJ cells transduced with Ha-rasV12 (Ras) or vector (WH) using an anti-c-Jun, anti-Ets1, or anti-c-Fos antibody or control IgG (rabbit IgG for the anti-c-Jun and anti-Ets1 antibodies and goat IgG for the anti-c-Fos antibody). DNA present in the immunoprecipitated complexes was quantified by real-time PCR using primers that amplify the region from −3086 to −2759 (A) or that from −7007 to −6723 (B) of the p38δ promoter. Fold enrichment was calculated by dividing signals from the antibody immunoprecipitates by that from the IgG immunoprecipitates. Values are means ± SDs for triplicates. *, P < 0.01 versus IgG by Student's t test.