Fig 8.

c-Jun, c-Fos, and Ets1 are activated by oncogenic ras through the MEK-ERK pathway during senescence induction. Numbers represent relative levels of proteins. (A) Western blot analysis of BJ cells transduced with Ha-rasV12 (Ras), constitutively active MEK1 (MEK1Q56P and MEK1E+), or vector (BP) on day 8 after ras transduction. (B) Western blot analysis of BJ cells transduced first with a dominant negative mutant of MEK1 (MEK1-AA) or vector (WH) and then with Ha-rasV12 (Ras) or vector (BP) on day 8 after ras transduction. (C) Western blot analysis of BJ cells transduced with Ha-rasV12 (Ras) or vector (WH) and treated with 5 μM U0126 or vehicle (Ctrl) on day 8 after ras transduction.