Fig 2.

Illustration of the Prdm4 cDNA-protein structural domain relationships. The Prdm4 transcript with numbered exons (top) is aligned with full-length Prdm4 protein and the recombinant and mutant proteins represented in this study. The protein that results from deletion of exons 9 to 11 (ΔZF) is shown, as well as the recombinant His-HA-tagged zinc finger domain protein used for SELEX and EMSA. Primer sites for cloning the zinc finger domain are shown as pink arrows. C-terminal EGFP (in outline) denotes tagged protein expressed in stably transfected ESCs, as used for ChIP-seq, Western blot analysis, and imaging. UTR, untranslated region.