Table 1.

Colocalization of SORLA and APP with marker proteins in SY5Y-A/S cells^a

Colocalization	Mean tM value ± SEM		P value
	With PACS1	Without PACS1
SORLA and Vti1b
tM1 (Vti1b-SORLA overlap)	0.254 ± 0.017	0.186 ± 0.015	<0.01
tM2 (SORLA-Vti1b overlap)	0.586 ± 0.023	0.430 ± 0.014	<0.001
SORLA and EEA1
tM1 (EEA1-SORLA overlap)	0.072 ± 0.004	0.117 ± 0.009	<0.01
tM2 (SORLA-EEA1 overlap)	0.152 ± 0.017	0.220 ± 0.017	<0.001
APP and SORLA
tM1 (SORLA-APP overlap)	0.504 ± 0.038	0.457 ± 0.039	>0.05
tM2 (APP-SORLA overlap)	0.233 ± 0.018	0.251 ± 0.002	>0.05
APP and Vti1b
tM1 (Vti1b-APP overlap)	0.134 ± 0.01	0.118 ± 0.007	>0.05
tM2 (APP-Vti1b overlap)	0.84 ± 0.016	0.611 ± 0.028	<0.001
APP and EEA1
tM1 (EEA1-APP overlap)	0.059 ± 0.004	0.081 ± 0.004	<0.001
tM2 (APP-EEA1 overlap)	0.448 ± 0.026	0.559 ± 0.027	<0.01

^aColocalization of SORLA and APP with the indicated proteins in SY5Y-A/S cells treated with a siRNA directed against endogenous PACS1 (without PACS1) or with a scrambled control siRNA (with PACS1) was tested by confocal immunofluorescence microscopy. The extent of colocalization was scored by determination of thresholded Manders (tM) values as detailed in Materials and Methods.