Fig 5.

Interference with Cdc42 function in HUVECs disrupted tube formation. (A through D) Representative images showing tube formation in HUVECs infected/transfected with a control green fluorescent protein (GFP)-tagged adenovirus (A), an adenovirus expressing HA-tagged dominant negative Cdc42-T17N (B), scrambled siRNA (C), or Cdc42-specific siRNA (D). (E) Quantitative analysis of tube length in HUVECs infected with a control GFP-tagged adenovirus or the HA-tagged Cdc42-T17N mutant adenovirus. *, P < 0.05. (F) Western blotting with anti-HA antibodies was carried out to confirm the overexpression of exogenous Cdc42. (G) Quantitative analysis of tube length in HUVECs transfected with scrambled or Cdc42-specific siRNA. (H) Western blotting showed that Cdc42 expression was lower in cells treated with Cdc42 siRNA than in control cells. Vinculin was utilized as a loading control.