Fig 9.
Inactivation of Cdc42 did not influence the cell cycle progression of HUVECs but impaired EC migration. (A through F) Cultured HUVECs were transfected with scrambled siRNA (A, B, and C) or Cdc42 siRNA (D, E, and F). Three days later, BrdU was added and was incubated for 18 h. BrdU (A and D) and VE-cadherin (B and E) staining was then performed. (C and F) Merged images. Bars, 50 μm. (G) Quantitative analysis of BrdU incorporation shows no significant difference between control and Cdc42 knockdown cells with or without fetal bovine serum (FBS) stimulation. (H) Cultured Cdc42flox/flox ECs were infected with Ad-LacZ, Ad-Cre, or Ad-Cre plus Ad-Cdc42 (wild type). The infected ECs were subjected to Boyden chamber analysis in response to VEGF (1 ng/ml). *, P < 0.05.