Fig 6.

Cell adhesion promotes increases in intracellular Ca²⁺. Aliquots of Fluo-4-AM-labeled Jurkat cells were seeded onto dishes coated with CS1, fibronectin (Fn), or BSA with or without EGTA added to the extracellular medium. Fluo-4-AM fluorescence were determined at the indicated times following cell seeding. Data plotted are means ± standard deviations (n = 5 replicate wells). P < 0.01 for t = 20 to 35 min for Fn versus BSA; the difference was not significant for t = 0 to 15 min.