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Increased intracellular oxidative stress in Cyp1b1−/− TM cells. (A) Increased DHE staining in Cyp1b1−/− TM cells (∗∗, P < 0.0001) (n = 4), which was prevented by the antioxidant NAC (∗∗, P = 0.003) (n = 4). Cyp1b1+/+ TM cells showed increased DHE staining when incubated with TMS (∗∗, P = 0.005) (n = 3). (B) Quantitative assessment of fluorescence intensities.
