Altered expression of ECM proteins in Cyp1b1−/− TM cells. (A) Decreased tenascin-C and Postn secretion into the CM of Cyp1b1−/− TM cells. (B) The decreased Postn secretion from Cyp1b1−/− TM cells was completely prevented in the presence of NAC. (C) Cyp1b1−/− TM tissue showed lower levels of Postn staining (arrows) than did Cyp1b1+/+ TM tissue. Scale bar, 25 μm. (D) Quantitative assessment of the fluorescent density shown in panel C (∗, P = 0.0332) (Cyp1b1+/+, n = 3; Cyp1b1−/−, n = 4). (E) Human glaucomatous TM (GTM) tissues also showed decreased Postn staining compared to normal tissue (NTM). SC, Schlemm's canal. Scale bars, 200 μm (top) and 25 μm (bottom). (F) Quantitative assessment of data in panel E (∗, P = 0.0291) (n = 4).