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. Author manuscript; available in PMC: 2013 Oct 29.
Published in final edited form as: J Virol Methods. 2012 Jun 6;185(1):10.1016/j.jviromet.2012.05.030. doi: 10.1016/j.jviromet.2012.05.030

Fig.2. Trypsin dependence of rhMPV-F and rhMPV.

Fig.2

LLC-MK 2 cells were infected by rhMPV-F or rhMPV and incubated with DMEM with or without 0.2μg/mL of trypsin-TPCK. Indirect immunostaining assay was performed at day 6 post-infection. After removal of cell culture medium, the cells were fixed in acetone: methanol (at the ratio of 3:2) and permeablized in PBS containing 0.4% Triton X-100. The cells were then probed with anti-hMPV N monoclonal antibody, followed by incubation with HRP-conjugated rabbit anti-mouse antibody. After incubation with AEC substrate chromogen, positive cells were visualized under light microscope.