Fig. 2.

(A) Low power image of NB through fluorescent filter to show VAChT immunoreactivity. Scale bar=400 µm. (B1 and B2) Higher power images of areas represented in left and right red boxes in (A), respectively. Arrowheads and roman numerals indicate locations of cells represented in C. Scale bar=100 µm. (C) High power images of individual retrogradely labeled putative cholinergic neurons indicated by arrowheads in B through various fluorescence filters to show tracers with which they were labeled and therefore to which subregions of PFC they project. Individual columns show the same cell through various fluorescence filters; individual rows show different cells through the same fluorescence filter. Scale bar=20 µm. NB, Nucleus basalis; RT, reticular nucleus of thalamus; VAChT, vesicular acetylcholine transporter; OFC, orbitofrontal cortex; mPFC, medial prefrontal cortex; ACC, anterior cingulate cortex.