Fig. 8.

(A) Low power image of LC through fluorescent filter to show galanin immunoreactivity. The area enclosed by the red box is shown at higher magnification in B, and through merged fluorescence channels in B2 to show locations of retrogradely labeled cells shown in C. Scale bar in A and B=200 µm. (C) High power images of individual retrogradely labeled LC neurons indicated by arrowheads in B through various fluorescence filters to show tracers with which they were labeled and therefore to which subregions of PFC they project Individual columns show the same cell through various fluorescence filters; individual rows show different cells through the same fluorescence filter. Scale bar=20 µm. LC, locus coeruleus; Me5, mesencephalic trigeminal nucleus; 4V, fourth ventricle; OFC, orbitofrontal cortex; mPFC, medial prefrontal cortex; ACC, anterior cingulate cortex.