Figure 4. Exendin-4 reduced H₂O₂-induced Min6 cell DNA fragmentation.

A, Apoptotic DNA ladder analysis. fter t-BHP (200 µM for 24 hours, as in Figure A2) and H₂O₂ (100 µM for 24 hours, as in Figure A4) treatment, the DNA was purified using an apoptosis DNA ladder kit and analyzed on agarose gel. Figures A3 and A5 represent Exendin-4 groups that were treated with t-BHP or H₂O₂ after treatment with Exendin-4 100 µM for 48 hours. Figure B is a summary of the results and includes the gray comparison among the control group, the H₂O₂ (100 µM for 24 hours) group and the Exendin-4 group. The data are expressed as the means±S.D. (n = 6). a, b and c indicate the respective comparisons (p<0.05).