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An absorbance microplate reader was used to analyze the loss of mitochondrial membrane potentials. The figure shows the time course of the t-BHP-induced loss of mitochondrial membrane potential. The data are expressed as the means±S.D. (n = 6). a, b, c, d, e and f indicate the t-BHP groups and Exendin-4 groups at different intervention times (p<0.05).
