Figure 8. Exendin-4 attenuates the release of cytochrome c from the Min6 cell mitochondria.

Figure A shows the effect of Exendin-4 on cytochrome c in Min6 cells. The cells were treated with or without Exendin-4 and then with t-BHP for different intervention times. The cells were then collected, and cytoplasmic mitochondrial fractions were prepared using a mitochondrial/cytosol fraction kit. The samples from each fraction were analyzed using Western blot for cytochrome c. Figure B presents the mitochondria. Figure C includes the group gray comparison (cytoplasm). The data are expressed as the means±S.D. (n = 6). a, b, c, d and e indicate the blank comparison and the t-BHP (200 µM for 24 hours) and Exendin-4 (100 µM for 48 hours) group comparisons (p<0.05).