. 2013 Oct 24;8(10):e77349. doi: 10.1371/journal.pone.0077349

Table 3. Allelopathic activity.

	T. weissflogii + A. tonsa 10 days cultures			T. weissflogii + A. tonsa 20 days cultures			Spent medium from T. weissflogii pure culture			Spent medium from T. weissflogii + A. tonsa 10 days cultures			Spent medium from T. weissflogii + A. tonsa 20 days cultures			Synechococcus sp. + A. tonsa 1 day culture			Spent medium from Synechococcus sp.			Spent medium from Synechococcus sp. + A. tonsa 1 day culture
	5 mM SO₄ ²	30 mM SO₄ ²		5 mM SO₄ ²	30 mM SO₄ ²		5 mM SO₄ ^2-	30 mM SO₄ ^2-		5 mM SO₄ ^2-	30 mM SO₄ ^2-		5 mM SO₄ ^2-	30 mM SO₄ ^2-		5 mM SO₄ ²	30 mM SO₄ ²		5 mM SO₄ ^2-	30 mM SO₄ ^2-		5 mM SO₄ ^2-	30 mM SO₄ ^2-
A. tonsa viability	✓	✓		†	†		✓	✓		✓	✓		†	†		†	†		✓	✓		†	†

Copepod mortality was tested in the presence of either Thalassiosira weissflogii or Synechococcus sp., or using spent media of the algal cultures (with or without the grazers) after the algal cells were filtered out. In experiments with spent media, the copepod was fed with T. suecica as “safe food”. No effect was observed on the ciliates. These tests were carried out in triplicate. All replicates gave the same results in the same time.

✓ no effect on copepod viability; † the copepods died (see text for details).