Skip to main content
. 2013 Oct 24;8(10):e77016. doi: 10.1371/journal.pone.0077016

Figure 5. Poly-A as the gap between two fusion partners.

Figure 5

Left panel: In a fusion RNA, two neighboring genes' sequences have three relationships, i.e. 1) with an overlapped sequence, 2) with an unmatchable sequence as a gap, or 3) directly joined. Right panel: The 1–160th nt of EST AU142287 are matched the 429–588th nt of the last exon of the POLDIP3 mRNA from chromosome 22, followed by a poly-A tail (boldfaced and underlined). Since the wild type form of this exon should have 2248 nt, the polyadenylation actually follows an early transcriptional termination. The 178–526th nt region (italicized grey) after the poly-A is part of the ND2 mRNA from mtDNA. The 527–836th nt sequence belongs to the first two exons of the GNB2L1 mRNA from chromosome 5, with the first 10 nt (underlined lowercase letters) alternatively initiated from the −10 bp of the GNB2L1 gene. The last 85 nt (underlined) have a few deletion mismatches to the first 88 nt of exon 2 of the GNB2L1. The last 5 nt (agngg) are unmatchable and might belong to the cloning vector.