Figure 3. Transfer of N. brasiliensis experienced B cells enhances immunity to N. brasiliensis independently of endogenous B cell populations.

N. brasiliensis infected μMT and BALB/c mice were re-infected with 500xL3 larvae and at day 5 post-secondary infection, the intestinal worm burdens was quantified (A). The possible role for IL-4Rα expressing B cells in boosting immunity independently of endogenous B cells was determined by transfer of B cells isolated from N. brasiliensis infected IL-4Rα^−/lox (WT B cells) or MB1^Cre IL-4Rα^−/lox (IL-4Rα^−/− B cells) into naïve μMT mice. These mice were then infected with 500xL3 N. brasiliensis and worm burdens quantified at day 5 post infection (B). Mediastinal lymph node CD3⁺CD4⁺ T cell populations IL-13 responses (C) were established by FACS staining. Results shown represent 2 independent experiments. n = 4–7 mice per group.