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. 2013 Oct 24;8(10):e77808. doi: 10.1371/journal.pone.0077808

Table 1. Oligonucleotide primers used in this study.

Primer Sequence (5′ → 3′)
auto Fa GAGAATTCAGTAGATAAACGTACCGATTGGA
auto R b GAGGATCCGCTGCTTTAGCATGGAATAGAG
ami Fa CTGAATTCCGCTGCCGGATTGTATATTA
ami R b CTGGATCCTAATCATAGTAGCGGAGGTGC
iap Fa TTGAATTCGCGGGCTATTTCTCGATACTG
iap R b TTGGATCCTGTAGCCGCGATAGTTGCT
murA Fa TAGAATTCGCCATCATACAAAGTGTTGC
murA R b TAGGATCCACCCCAGCAATTGTTGCACC
spl Fa TCGAATTCGAAGTTAGTGACAAGCTCGC
spl R b TCGGATCCTGCTGCGAGTGAGATCGC
ftsEX-spl Fa GTGAATTCGTGCAAGTGAAGTAATGGGTGA
ftsEX-spl R b GTGGATCCGGCAGCTGTTATGCCGTTAG
dltABCD Fc GCGCCCGGGACAAGCCTTTTCTCCTGCA
dltABCD Rb CGGGATCCAGGGAAATCCGGTGTCTTCT
secA2 Fa TCGAATTCTGTAGCCGCGATAGTTGCT
secA2 R b TAGGATCCGCGGGCTATTTCTCGATACTG
a

The sequence in boldface type is the EcoRI restriction enzyme site.

b

The sequence in boldface type is the BamHI restriction enzyme site.

c

The sequence in boldface type is the SmaI restriction enzyme site.