Table 1. Oligonucleotide primers used in this study.
Primer | Sequence (5′ → 3′) |
auto Fa | GAGAATTCAGTAGATAAACGTACCGATTGGA |
auto R b | GAGGATCCGCTGCTTTAGCATGGAATAGAG |
ami Fa | CTGAATTCCGCTGCCGGATTGTATATTA |
ami R b | CTGGATCCTAATCATAGTAGCGGAGGTGC |
iap Fa | TTGAATTCGCGGGCTATTTCTCGATACTG |
iap R b | TTGGATCCTGTAGCCGCGATAGTTGCT |
murA Fa | TAGAATTCGCCATCATACAAAGTGTTGC |
murA R b | TAGGATCCACCCCAGCAATTGTTGCACC |
spl Fa | TCGAATTCGAAGTTAGTGACAAGCTCGC |
spl R b | TCGGATCCTGCTGCGAGTGAGATCGC |
ftsEX-spl Fa | GTGAATTCGTGCAAGTGAAGTAATGGGTGA |
ftsEX-spl R b | GTGGATCCGGCAGCTGTTATGCCGTTAG |
dltABCD Fc | GCGCCCGGGACAAGCCTTTTCTCCTGCA |
dltABCD Rb | CGGGATCCAGGGAAATCCGGTGTCTTCT |
secA2 Fa | TCGAATTCTGTAGCCGCGATAGTTGCT |
secA2 R b | TAGGATCCGCGGGCTATTTCTCGATACTG |
The sequence in boldface type is the EcoRI restriction enzyme site.
The sequence in boldface type is the BamHI restriction enzyme site.
The sequence in boldface type is the SmaI restriction enzyme site.