Table 3. Cell length and cell wall thickness of L. monocytogenes strains.
Strain | Average cell length (µm) ± SDa | Average cell wall thickness (nm) ± SDb | ||
BHI medium | BHI medium + PenG | BHI medium | BHI medium + PenG | |
EGD | 1.52 ± 0.25 | 1.35 ± 0.22c | 26.52 ± 1.82 | 30.78 ± 2.86c |
Δ fri | 2.02 ± 0.39d | 2.37 ± 0.76c,d | 25.59 ± 1.67 | 18.81 ± 2.49c,d |
Mean cell lengths were determined by measuring 100 cells of each strain grown in the presence (+ PenG) or absence of 0.09 µg/ml penicillin G. The analysis was performed using two independently prepared samples. From each independent sample, equal numbers of cells were analyzed ± standard deviation (SD).
Mean cell wall thickness was determined by measurement of 20 cells of each strain grown in the presence (+ PenG) or absence of 0.09 µg/ml penicillin G. The analysis was performed using two independently prepared samples. From each independent sample, equal numbers of cells were analyzed ± standard deviation (SD).
Significant differences following growth in the presence and absence of penicillin G (Student's t-test; P<0.05).
Significant differences between the studied strains (Student's t-test; P<0.05).