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. 2013 Oct 24;9(10):e1003650. doi: 10.1371/journal.ppat.1003650

Figure 2. Pharmacologic inhibition of viral replication inhibits onset of autoimmune diabetes.

Figure 2

(A–B) C57BL/6 mice were treated intraperitoneally with Ribavirin (5 mg daily), starting on day −3. On day 0, mice were infected with 200 PFU LCMV. LCMV titers in the spleen were measured by plaque assay on days 1 and 2 after infection (n = 5–7, A). Levels of IFN-α was measured in the serum by ELISA (n = 4–5, B). (C–D) RIP-GP mice were treated intraperitoneally with Ribavirin (5 mg daily), starting on day −3. On day 0, mice were infected with 200 PFU of LCMV-WE. Numbers of islet-specific CD8+ T cells were determined by tetramer staining and flow cytometry (C, n = 4). The onset of diabetes was assessed by measuring serum glucose concentrations at the indicated time points (D, n = 9). *** P<0.001 (Student's t-test) (A and B), two-way analysis of variance (ANOVA) (C) or Log-rank (Mantel-Cox) (D).