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HIV-1 sequence from −7 to −34 from circulating HIV-1 strains from major and outlier clades, simian immunodeficiency viruses from Chimpanzee, African green monkey, Mandrill, Macaque, HIV-2, feline immunodeficiency virus, equine infectious anemia virus and bovine immunodeficiency virus were aligned to the HIV-1 NL43 strain used in this study as described in materials and methods. Conserved sequence shown as dashes, changes from NL43 are indicated.
