Table 1. Real-time PCR primers.

Target genea	Oligo Name	orientationb	Sequence 5′ to 3′c	Binding positiond
HIV U3/R	OJWB904	sense	GAGCTTGCTACAAGGGACTTTC	−116
	OJWB809	antisense	CAGGCTCAGATCTGGTCTAAC	25
fLuc	OJWB891	sense	TGTACACGTTCGTCACATCTCATC	9291
	OJWB892	antisense	TTATCAGTGCAATTGTTTTGTCACG	9386
gLuc	OJWB820	sense	AGTGTTCTGACCTGCTCAAGA	536
	OJWB821	antisense	AGTCACCACCGGCCCCCTTGA	637
HIV-R	OJWB694	sense	GGGTCTCTCTGGTTAGACCAG	1
	OJWB695	antisense	GGGTTCCCTAGTTAGCCAGAG	58
gag	OJWB872	sense	CGAGGATTGTGGAACTTCTGGGACGC	8556
	OJWB873	antisense	TTATAGCAAAATCCTTTCCAAGCCCTGTC	8791
fLUC	OJWB874	sense	AGGCGAATTATGTGTCAGAGGACCTATG	9961
	OJWB875	antisense	GTTGTAACAATATCGATTCCAATTCAGCG	10191
vif	OJWB910	sense	ATGGCAGGTGATGATTGTGTG	5052
	OJWB911	antisense	CCATGTGTTAATCCTCATCCTGTC	5103
ZASC1	OJWB1005	sense	CTACCTGGTTCCCAGTCCTGTA	NA
	OJWB1006	antisense	GCTGGTTGACTGATTCAGGTGAG
Actin	OJWB887	sense	TCACCCACACTGTGCCCATCTACGA	NA
	OJWB888	antisense	CAGCGGAACCGCTCATTGCCAATGG

^aThe gene or viral component that the primer amplifies.

^bDenotes whether the oligo binds on the coding strand (relative to the mRNA) or noncoding strand.

^cSequence of the oligo written in the 5′ to 3′ orientation.

^dThe region of amplification, relative to the HIV-1 transcription start site in construct.