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. Author manuscript; available in PMC: 2013 Oct 29.
Published in final edited form as: Cell Rep. 2013 Jan 10;3(1):10.1016/j.celrep.2012.11.029. doi: 10.1016/j.celrep.2012.11.029

Figure 4. Mitochondrial Gene Expression and Respiratory Function during Iron Deprivation and Recovery.

Figure 4

(A) Experimental workflow for the analysis of mitochondrial gene expression and respiration during DFO response and recovery.

(B) Level of the indicated proteins in C2C12 myoblasts treated with 100 μM DFO for 24 hr then passaged every 24 hr in DFO-free media as assessed by immunoblotting.

(C) Mitochondrial respiratory profile of untreated myoblasts at day 1 of analysis. Data are displayed as mean ± SD of 14–16 replicates.

(D–F) Basal OCR (pmol/min)/cell number (D), spare respiratory capacity (E), and coupling efficiency (F) of myoblasts treated with 100 μM DFO for 24 hr then passaged every 24 hr in DFO-free media. Data are displayed as mean±SD of 14–16 replicates (*p<0.05, Student's t test in D, ANOVA with Tukey's test in E and F).

See also Figure S4.