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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nature. 2013 Jul 24;500(7460):77–80. doi: 10.1038/nature12403

Figure 1. Procotyla fluviatilis fails to regenerate heads after amputation in posterior body regions.

Figure 1

a–b, Proficient and failed head regeneration 10 days after amputation in Reg+ regions Reg− regions respectively (n=25/25). Dashed lines indicate amputation planes. c–d, Complete wound epithelium (arrows; n=8/9) and full wound closure (n=7/8) 48 hours after amputation in Reg− tissue. Scale bar in d, 100 µm. e–f, Mitotic activity 4 days after amputation in Reg+ and Reg− tissues (n=10/treatment). g–j, Pf-ndk and Pf-sFRP1 expression 24 hours after amputation in Reg+ and Reg− tissues (n=8/treatment). Anterior is to the left. Scale bars, 250 µm unless otherwise noted.