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. 2013 Oct 24;9(10):e1003908. doi: 10.1371/journal.pgen.1003908

Figure 5. LRO autofluorescence and vital dye accumulation are regulated by skat-1.

Figure 5

(A) A kat-1 mutant has high blue spectrum autofluorescence, which is concordant with the high LRO Nile red phenotype. Mutation of skat-1 in kat-1 mutants fully suppresses accumulation of LRO blue spectrum autofluorescence with age. kat-1(mg368) and kat-1(mg449) show similar phenotypes in blue spectrum autofluorescence. (B) kat-1 and skat-1 both show elevated green spectrum autofluorescence that is synergistic in the kat-1;skat-1 double mutant. (C) Green autofluorescence in a representative day 1 adult animal for each genotype. (D) C1-BODIPY-C12, which is accumulated in the LRO, is also increased in kat-1 mutants and fully suppressed like Nile red in the kat-1;skat-1 double mutant. (E) In contrast, LysoTracker Red is decreased in kat-1 and skat-1 single mutants and synthetically decreased in the kat-1;skat-1 double mutant. (N>25; *, P<0.05; **, P<0.0001 by one-way ANOVA with Bonferroni correction.)