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. 2013 Oct 24;7(10):e2482. doi: 10.1371/journal.pntd.0002482

Figure 8. SmCD59.1 and SmCD59.2 gene expression knockdown and complement mediated killing assay of suppressed parasites.

Figure 8

(A) Relative SmCD59.1 and SmCD59.2 gene expression (mean ± SE) in schistosomula 5 days after treatment with SmCD59 siRNAs or control siRNA (control) or not treated parasites (none). “SmCD59.1+2” indicates parasites treated with a mixture of equal molar amounts of SmCD59.1 and SmCD59.2 siRNAs. (B) Protein levels in SmCD59-suppressed and control schistosomula groups as described in (A). Western blot results are shown for SmCD59.1 (top panel), SmCD59.2 (middle panel) and the S. mansoni aquaporin (SmAQP) as a loading control (bottom panel). (C) The parasites were treated with immune rat serum (IRS), normal rat serum (NRS), or kept in medium only and then treated with normal human serum (NHS) as source of complement. Percent net mortality of schistosomula is shown and was determined by background mortality subtraction of parasites treated with heat-inactivated NHS. Results shown are mean ± SD of duplicate samples in three independent experiments. (D) Freshly transformed schistosomula (3 h in culture) were included as a positive control in the complement mediated killing assay.