Figure 2. MLH1 shRNA expression is inducible and reversible.

NCI-H23 subclones 4-10 and 4-13 that were induced for MLH1 shRNA were split into two cultures, one maintained in inducing conditions (+ doxycycline) and the other grown in the absence of doxycycline (-) to allow re-expression of MLH1. Protein lysates were prepared and analyzed by SDS-PAGE followed by immunoblotting for expression of the MLH1 protein. Tubulin was used as a control for equal protein loading across samples.