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. 2013 Oct 29;8(10):e77870. doi: 10.1371/journal.pone.0077870

Figure 2. Expression of Flag-Dmp1 mRNA and proteins in tissues of MDTG mice.

Figure 2

A. Expression of Flag-Dmp1 mRNA in tissues from a mouse of MDTG (12-week-old, strain 76) as studied by real-time PCR. The level of Flag-Dmp1 in lactating mammary glands was defined as 1.0. B. Expression of the Flag-Dmp1 protein in tissues from a MDTG mouse (12-week-old, strain 76). Proteins were extracted in ice-cold EBC buffer (19) and 100 µg of proteins were analyzed by Western blotting with the antibody to the FLAG tag.