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. 2013 Oct 29;8(10):e77870. doi: 10.1371/journal.pone.0077870

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© 2013 Fry et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Total tumor weight harvested at sacrifice. The amount of tumors recovered at sacrifice (4 weeks after the first tumor was found) was significantly decreased in all three transgenic strains. B. Western blotting analyses of proteins expressed in MDTG;neu tumors. C. Mutually exclusive expression of Flag-Dmp1 and Ki67 in a MDTG;neu tumor (strain 76, #3987). Flag-Dmp1 was stained brown; Ki67 was stained blue. Scale bars show 100 µm. D. Gene copy number assay for Flag-Dmp1 in paired tails and MDTG;neu tumors showing partial deletion of the transgene in half of the cases.