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. 2013 Oct 29;8(10):e78236. doi: 10.1371/journal.pone.0078236

Figure 2. Stem cell features of NG2-glia.

Figure 2

Mice were treated with BrdU in the drinking water for 28 days before sacrifice to mark proliferating cells. Sox2 (green), NG2 (red) and BrdU (blue) mark overlapping populations of cells (A, box enlarged in B). NG2+/BrdU+ cells were found either Sox2+ (yellow arrowheads in B) or showed no or barely detectable immuno-signal for Sox2 (denoted as Sox2-, white arrows in B). As NG2-glia are usually found in a well-spaced tiled pattern, pairs of adjacent NG2-glia are likely to represent recently-divided cells. In NG2 doublets, Sox2 was appreciable (C), absent or barely detectable (D), or limited to one cell (E; white arrowheads indicate nascent NG2-glial cell pair). (F), Quantification of the proportion of BrdU+ cells that also expressed NG2 (bar, left), the number of NG2-glia cells that also expressed Sox2 (bar, middle), and the number of Sox2+/BrdU+ cells that also expressed NG2 (bar, right); n = 3 mice.