Figure 6. Effects of GAEE on Rho GTPases expression and actin assembly.

(A) Cells were treated with indicated concentrations of GAEE for 24 h, the downregulation of RhoA, Rac1, and Cdc42 was observed, and the expression of N-WASP was not affected. (B) MDA-MB-231 cells were treated with GAEE (10 or 20 µg/ml) for 24 h, and then cell lysates were prepared and subjected to immunoprecipitation with N-WASP, and followed by immunoblotting with Cdc42 and actin. The samples were blotted with anti-N-WASP as a control.