Table 1. Primers used for bisulfite mapping and ChIP of mir-496.

Name	Sequence
Outer bisulfite mir-496 forward −184..−162	TGGGTGGTGTGTTGttAttTTt
Outer bisulfite mir-496 reverse −402..−423	TCCATTCAaCCAaaAaTTCCTT
Nested bisulfite mir-496 forward −83..−61	TGGAGGTTGTttATGGTGTGTT
Nested bisulfite mir-496 reverse +368..+389	CCACACAACCAAAATAATTTCA
Outer pcpgl bisulfite mir-496-pcpgl forward	TTAAAAGGAATTttTGtAGGAtTAG
Outer pcpgl bisulfite mir-496-pcpgl reverse	TTTCTTAATATTCTTaaCATCCTCCA
Nested bisulfite mir-496-pcpgl forward	TTTTTTGAATGGTTTTTTGTAAGAG
Nested bisulfite mir-496-pcpgl reverse	AAAAAAAATTAACCATATAATACTCATCAT
Pyrosequencing mir-496-pcpgl S1	AGTAAGGGATGGAGT
Pyrosequencing mir-496-pcpgl S2	TGTTGTTATTTTTTTGATTTTTAGT
ChIP mir-496 −11..+264 for	GGAAGCGAGCACCCAAGT
ChIP mir-496 −11..+264 rev	CATGTCAACTAAAACGTCAGCA
ChIP mir-496 −834..−550 for	GGGTCTGCGCTAGCGTGT
ChIP mir-496 −834..−550 rev	AAGCTCCACTTCTTCCCCAAA
mir-496-pcpgl ChIP forward	GGAAGCGAGCACCCAAGT
mir-496-pcpgl ChIP reverse	TGCCATCTTCCAGAGGGTAG
luc-pcpgl ChIP forward	TCCCTGAAGTTGGTGGAGAC
luc-pcpgl ChIP reverse	GCAGGTGTGGTCAGAGATGA
qPCR MBD2 forward	CAAAGTCACAAATCTCCTAGTAAAGT
qPCR MBD2 reverse	TATAATTTGTTCTGTTACATCTGATACACT
qPCR CTSH forward	TACTGGCTGTTGGGTATGGAG
qPCR CTSH reverse	CGATGAGGAAGTACCCGTTC
qPCR POU2F3 forward	ACTCCAAAGCAGCAGTGAAC
qPCR POU2F3 reverse	CGGTACCAAGATCCTGAAGAG
qPCR PTGS1 forward	CGTAGGAGAGAAGGAGATGG
qPCR PTGS1 reverse	AGAAGCAGTCCAGGGTAGAAC

Expression of targets and MBD2.