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. 2013 Oct 29;8(10):e77611. doi: 10.1371/journal.pone.0077611

Figure 7. Atrioventricular (AV) endocardial cushion explant culture from E10.5 NOS3−/− and WT mice.

Figure 7

(A) Spindle shaped mesenchymal cells (arrows) were formed after 24 hours of culture. Lower panel is an enlargement of the boxed area. (B) The total number of mesenchymal cells per mm of explant cushion tissue was significantly decreased in NOS3−/− compared to WT explants. Data are mean ± SEM from 6 explants per group. *P<0.05 vs. WT(NOS3+/+). Black scale bar = 100 µm, white scale bar = 10 µm. (C) Proposed signaling pathway by which NOS3 promotes endocardial EndMT and AV valve development.