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. 2013 Jul 18;32(4):775–784. doi: 10.3892/ijmm.2013.1449

Figure 4.

Figure 4

Effects of the phosphatidylinositol 3-kinase PI3K inhibitor (LY294002) and Akt inhibitor (triciribine) on H-ras-induced matrix metalloproteinase-2 (MMP-2) activity, and on the migration and invasion of MCF10A cells. (A and B) Cells were starved in serum-free DMEM/F12 for 24 h and then treated with samples at the indicated concentrations for 24 h. The conditioned medium was analyzed by zymography, as described in Materials and methods. (C) Invaded cells were quantified by counting the cells that had migrated to the lower side of the filter in random microscopic fields. Each bar indicates the mean ± SD (n=3). *p<0.05, **p<0.01 compared to the control. (D) The confluent H-ras MCF10A cells in serum-free medium were treated with 40 μM LY294002 or 20 μM triciribine. Thereafter, the widths of the injury lines were measured at 0, 12, 18 and 24 h. Results are expressed as the widths of the injury lines relative to untreated controls at 0 h, as determined from three independent experiments. Data are the means ± SD.