Figure 3.

NtBuHA prevents H₂O₂-induced apoptosis in INCL lymphoblasts. (Panel a) Flow cytometric analysis of apoptotic cells using apoptosis-marker, annexin V and necrotic cells using necrotic cell marker PI: Untreated control INCL lymphoblasts (Upper left panel); NtBuHA-pretreated (Upper middle panel); and NtBuHA pre- as well as co-treated (Upper right panel). INCL lymphoblasts were either untreated and exposed to oxidative-stress by H₂O₂ (untreated control, Lower left panel) or the cells were first pretreated with NtBuHA for 12h and then incubated with H₂O₂ in the absence (Lower middle panel) or presence of NtBuHA (Lower right panel) for 3h. (Panel b) Graphical presentation of level of apoptotic cells in untreated and NtBuHA-treated INCL cells. Apoptotic cells represent annexinV positive cells detected by FACS (Summation of cell number in Q2 + Q3).