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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Biochemistry. 2013 May 7;52(20):10.1021/bi400230u. doi: 10.1021/bi400230u

Figure 2.

Figure 2

A. Expansions of the region containing the SMR of 1D 13C-edited 1H-NMR spectra of 3 µM 15N,13C-labeled C2AB fragment acquired in the presence of 1 mM EDTA (-Ca2+) or 1 mM Ca2+ (+Ca2+), and in the absence (−SC) or presence (+SC) of 3.5 µM SNARE complex. B. analogous expansions of 1D 13C-edited 1H-NMR spectra of 3 µM 15N,13C-labeled C2AB fragment in the presence of 3.5 µM SNARE complex and the indicated concentrations of Ca2+ in µM units. C. Plot of the normalized SMR intensities observed in 1D 13C-edited 1H-NMR spectra of 3 µM 15N,13C-labeled C2AB fragment in the presence of 3.5 µM SNARE complex and variable concentrations of Ca2+. A subset of the data corresponding to this titration is shown in panel B. The curve shows the fit to a Hill equation.