Human mid-trimester amniotic fluid stem cells cultured under embryonic stem cell conditions with valproic acid acquire pluripotent characteristics.
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Moschidou D73
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2013
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“VPA treatment significant induces up-regulation of OCT4 (75 ± 12.5%), SOX2 (20.8 ± 4.4%), KLF4 (21 ± 388 3.2%) and C-MYC (32100 ± 320%) compare to non-treated cells.
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Valproic Acid Confers Functional Pluripotency to Human Amniotic Fluid Stem Cells in a Transgene-free Approach.
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Moschidou D18
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2012
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“VPA led to an upregulation levels of: OCT4 (from 10.2 ± 0.6 to 79.6 ± 18.30%), NANOG (from 12.2 ± 1.2 to 85.3 ± 5.3%), SOX2 (from 55 ± 8.2 to 164± 22.3%), KLF4 (from 360 ± 19.5 to 705.4 ± 16.2%) and c-MYC (from 26,950 ± 750 to 34,200 ± 350%).”
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Generation of human β-thalassemia induced pluripotent stem cells from amniotic fluid cells using a single excisable lentiviral stem cell cassette.
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Fan Y76
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2012
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“The efficiency for generation of iPS was approximately 0.33% in human β-thalassemia AF cells and approximately 0.02% in human β thalassemia skin fibroblast cells.”
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Amniotic Fluid Cells Are More Efficiently Reprogrammed to Pluripotency Than Adult Cells.
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Galende E74
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2010
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“AF skin cells formed iPS colonies approximately twice as fast as cultured adult skin.”
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Generation of Human Induced Pluripotent Stem Cells from Umbilical Cord Matrix and Amniotic Membrane Mesenchymal Cells.
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Cai J77
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2009
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“up to 0.4% of reprogramming efficiency in iPSCs from mesenchymal cells of umbilical cord matrix; up to 0.1% efficiency in iPSCs from placental amniotic membrane.”
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| Pluripotency can be rapidly and efficiently induced in human amniotic fluid-derived cells. |
Li C63
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2009 |
“frequencies for induction of pluripotency in hAFDCs were between 0.059% and 1.525%; all selected iPS colonies hAFDC-derived were OCT4 positive and 90.5% were NANOG positive” |
