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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Amino Acids. 2013 Jul 3;46(3):531–542. doi: 10.1007/s00726-013-1531-z

Figure 2.

Figure 2

Effect of stable knockdown of SMO on H. pylori-stimulated iNOS expression and NO production. (A–C) RAW 264.7 cells stably transfected with Scr shRNA or SMO shRNA were activated with HPL at an MOI of 100 for 24 h. (A) mRNA expression assessed by RT-PCR at 6 h after stimulation with HPL. (B) Western blot analysis for iNOS (130 kDa) and β-actin (42 kDa) 24 h after stimulation. In A and B, data are representative of 3 separate experiments. (C) NO2− levels, measured by Griess assay. (D) Peritoneal macrophages were transiently transfected with either Scr siRNA or SMO siRNA, and NO2− levels were measured 72 h after activation with HPL. For C and D, ***p < 0.001 vs unstimulated control cells transfected with Scr shRNA or siRNA, and §§§p < 0.001 vs HPL-stimulated cells transfected with Scr shRNA or siRNA. Data are from 3 experiments.